Oqsillar va peptidlar kimyosi laboratoriyasi

Rahbar: kimyo fanlari doktori

Oshepkova Yuliya Igorevna

e-mail: joshepkova05@rambler.ru

Oqsillar va peptidlar kimyosi laboratoriyasi 1973 yilda zoofitotoksinlar laboratoriyasi negizida tashkil topgan bo‘lib, O‘zRFA Bioorganik kimyo bo‘limining bo‘linmalaridan biri hisoblangan. Laboratoriya faoliyati davomida tabiiy birikmalar, toksinlar, fermentlar va boshqa biologik faol komponentlarning tuzilishi va funktsiyasi o‘rganilgan. O‘rta Osiyo hayvonlari zaharidan birinchi bo‘lib toksik ta’sirga ega 50 dan ortiq fiziologik faol oqsillar va peptidlar ajratib olingan, ulardan 15 tasining kimyoviy birlamchi tuzilishi aniqlangan. O‘rgimchak Lactrodectuszaharidan oqsil tabiatga ega bo‘lgan murakkab to‘rtlamchi tuzilishli, molekulyar massasi 75000 Da ga teng to‘rtta subbirlikdan tashkil topgan neyrotoksin ajratib olingan (Toshmuxamedov M.S., Qosimov A.A., Odilbekov M.T., Salixov Sh.I.,). Turli bo‘g‘imoyoqlilar zaharidan ajratilgan yuqori spetsifiklikka ega bo‘lgan toksinlarni tadqiq qilish ularning ta’sir mexanizmini o‘rganish va ulardan neyrofiziologik tadqiqotlar uchun “asbob” sifatida foydalanish imkonini berdi (Salixov Sh.I., Qosimov A.A.). O‘rganilgan qator yuqori spetsifik neyrotoksinlardan foydalanish biologik membrananing strukturaviy va molekulyar xususiyatlari bilan bog‘liq bo‘lgan tirik hujayrada sodir bo‘ladigan muhim hayotiy jarayonlarni anglab yetishga olib keladi. Tadqiqotlarning amaliy yakuni o‘laroq ajratib olingan neyrotoksinlardan antitela sifatida foydalangan holda kobra, chayon va qoraqurt zaharlariga qarshi antizardob yaratishdan iborat edi (Salixov Sh.I., Qosimov A.A., Inog‘amov O‘.Q.).

Bir qator kichik molekulali toksinlarning turli patologik holatlarda: surunkali buyrak yetishmovchiligi, travmatologiya, turli virusli kasalliklarda tuzilishi o‘rganildi. Gemodializ jarayonlarini dasturlashni ta’minlashga imkon beruvchi kichik molekulali toksinlar kontsentratsiyasini miqdoriy aniqlashning ekspress usuli ishlab chiqildi (Muxamadieva Sh.G., Kadrova A.F., Sultanova E.M.). sorbentlar bilan toksin kon’yugatlari asosida immunosorbentlar olindi (Qosimov A.A., Salixov Sh.I.). O‘zbekiston Respublikasi tibbiyot hodimlari bilan hamkorlikda ishlar olib borildi va ular asosida tibbiyot soxasida nomzodlik va doktorlik dissertatsiyalari himoya qilindi.

1984 yilda laboratoriya faoliyati o‘simlik va hayvonlar organizmidan ajratib olingan oqsillar va peptidlarni tadqiq etishga, shuningdek, g‘o‘zaning o‘sish va rivojlanish jarayonlarida kechadigan biokimyoviy jarayonlarni o‘rganishga yo‘naltirildi. G‘o‘zaning gormonga bog‘liq oqsillarining tuzilishi va funktsiyasi uning o‘sish va rivojlanish jarayonida o‘rganildi. G‘o‘zadan fitogormon retseptorlari ajratildi, fizik-kimyoviy xossalari o‘rganildi va ularning g‘o‘za barglarini to‘kilishidagi regulyatorlik roli isbotlandi. G‘o‘zada defoliatsiya paytida sodir bo‘ladigan jarayonlarning molekulyar mexanizmi tadqiq qilindi. Ushbu tadqiqotlar asosida defolirlovchi va o‘sishni stimullovchi faollikka ega bo‘lgan birikmalar skriningi kriteriyalari ishlab chiqildi. (Veshkurova O.N., Salixov Sh.I.).

Laboratoriyada fan doktori ilmiy darajasini olish uchun 7 ta dissertatsiya himoya qilindi; Dolimbek Bexzod Ziyotbek (Texas Universiteti professori), Abulimiti Yili (XXR FA Shinjon fizika va kimyo texnika instituti direktor o‘rinbosari) shular jumlasidandir.

Laboratoriya tashkil etilgandan beri unga akademik, b.f.d. Salixov Sh.I. (1973 yildan 2017 yilgacha.) va k.f.d. Oщepkova Yu.I (2018 yildan) rahbarlik qilib kelmoqdalar.

2018 yilda Institutni qayta tashkillashtirish natijasida Leontev V.B. (1973 yildan 1999 yilgacha), prof. Sodiqov A.A. (1999 yildan 2005 yilgacha) va  k.f.n. Sagdiev N.J. (2005 yildan 2017 yilgacha) rahbarlik qilgan bioregulyatorlar laboratoriyasi laboratoriya tarkibiga kiritildi. Professor Leontev V.B. rahbarligi ostida rekonstruktsiyalangan nukleoproteid komplekslari olindi, yadroviy oqsil gistonlar, giston bo‘lmagan oqsillar, HMG – in vitro va nativ xromatinda transkriptsiyani regulyatsiyalovchi oqsillar roli o‘rganildi. Yadro oqsillari va ular genomining funktsional jihatdan ahamiyatli hududlari kimyoviy tuzilishini o‘zgarishida allosteriya va kooperativ tabiatning konformatsion o‘zgarishlarining muhim roli aniqlandi. (Mangutova Yu.S., Faynboym A.M., Leontev V.B.). Hujayra yadrosining oqsil biosintezidagi roli,. (Saitmuratova O.X., Leontev V.B.), shuningdek, yadro ichidagi biosintez mexanizmi va asab to‘qimalari yadroviy va neytral oqsillarining funktsional rolini aniqlashga bag‘ishlangan  qator tadqiqotlar olib borildi. Vaznsizlik sharoitida poliakrilamid geli sintezi bo‘yicha tajribalar taklif etildi va amalga oshirildi.  Tarmoqlangan tuzilishning xossalari tortishish kuchiga bog‘liqligi aniqlandi (Sodiqov A.A., Leontev V.B., Grechko G.M.).

Laboratoriyada yuqori texnologiyalardan biri – monoklonal antitelalar olish bo‘yicha gibrid biotexnologiya o‘rnatildi (Xashimova Z.S., Mangutova Yu.S., Suslo M.E., Leontev V.B.). KML (sichqon terisi o‘smasi), Akat (sichqon ingichka ichagi o‘smasi) va sarkoma S- 180 kabi ko‘chirib o‘tkaziladigan rak hujayralari kulturalari olindi; ushbu hujayra tizimlari asosida biologik faol moddalar, shu jumladan, o‘smaga qarshi vositalar skriningi uchun test-tizim yaratildi. (Kuznetsova N.N., Mardanova Z.I., Leontev V.B., Xashimova Z.S., Sodiqov A.A.), shuningdek, KML hujayra liniyasidan foydalangan holda metastazlash modeli olindi.

So‘nggi yillarda Oqsillar va Peptidlar Kimyosi laboratoriyasida o‘simlik va hayvonlar bioregulyatorlarini struktur-funktsional xarakterlash bo‘yicha tadqiqotlar olib borildi va ular asosida tibbiyot va qishloq xo‘jaligi sohasida muvaffaqqiyatli qo‘llanilib kelinayotgan bir qator preparatlar yaratildi.

 

Laboratoriyaning asosiy ilmiy yo‘nalishlari:

o‘simlik va hayvonlar antimikrob peptidlari va bioregulyatorlarining struktur-funktsional xarakteristikasi va ta’sir mexanizmi tadqiqi, oqsil-peptidli bioregulyatorlar olish texnologiyasini ishlab chiqish va ular asosida tibbiyot va qishloq xo‘jaligi soxalari uchun preparatlar yaratish, barqarorlik namoyon qiluvchi, prolongirlangan ta’sirga ega, biomavjudligining samaradorligi orttirilgan makromolekulyar terapevtik tizimlarni konstruktsiyalash.

 

 

Ilmiy yo‘nalish:

• Markaziy Osiyo o‘simlik va hayvonlarining oqsil-peptid tabiatli fiziologik faol moddalarining tadqiqi.
• o‘simlik va hayvonlar oqsil-peptidli bioregulyatorlari asosida yangi dorivor vositalar yaratish va ishlab chiqarish.
• barqarorlik namoyon qiluvchi, prolongirlangan ta’sirga ega, biomavjudligining samaradorligi orttirilgan makromolekulyar terapevtik tizimlarni konstruktsiyalash.

 

Eng muhim natijalar:

• antimikrob peptidlar va PR-oqsillarning o‘simlik patogen zamburug‘lariga chidamliligi o‘rganildi, bir qator tsisteinga boy peptidlar individual tarzda ajratib olindi, ularning qisman N-oxiri aminokislota ketma-ketligi aniqlandi;
• gossipol va qator terpenoidlarning g‘o‘za zararkunanda hasharotlari Heliothis oilasi uchun toksik ta’siri molekulyar mexanizmi tadqiq qilindi;
• dorivor preparatlarning bioxavfsizligi va bioekvivalentliligini farmakokinetik baholash bo‘yicha sinov markazi tashkil etildi;
• Ns-D2 defenzini va BucaIT insektotoksinining rekombinat shakllari olindi;
• sut emizuvchilar organlari regulyator peptidlari asosida yallig‘lanish kasalliklarini davolash uchun “Aflan”, tromblar hosil bo‘lishini oldini olish va davolash uchun “Kardin” dorivor preparatlari yaratildi;
• mexanik yaralar va termik kuyishlardan keyingi yiringli-septik asoratlarni davolash uchun “Sedanil” farmatsevtik kompozitsiyasi yaratildi;
• onkologik kasalliklar sut bezi o‘smasi, melanoma, to‘g‘ri ichak va endometriy o‘smalarini kompleks davolash uchun tavsiya etilgan “Biokor” –immunomodulyator preparati yaratildi;
• osteoporoz, yurak qon-tomir kasalliklari, sut bezi, endometriy, tuxumdon o‘smasi kasalliklari va menopauza simptomlarini davolash uchun “Soyaflan” –immunoreabilitatsiya tipidagi preparat yaratildi;
• o‘simlik yog‘laridan fermentativ usulda biodizel olishning samarali sxemasi ishlab chiqildi;
• tabiiy ob’ektlardan fiziologik faol moddalarni ajratish uchun mahalliy xom-ashyolar asosida ion-almashinuvchi, gidrofob va affin sorbentlar sintez qilish usullari ishlab chiqildi;
• sichqonlar ingichka ichagi o‘smasidan yangi ko‘chirib o‘tkaziladigan xujayra kulturasi ajratib olindi.

 

Natijalarning amaliy ahamiyati:

• O‘zbekiston Respublikasi farmsanoat korxonalarida yaratilayotgan original va generik preparatlarning bioekvivalentligi bo‘yicha farmakokinetik tadqiqotlar olib borilmoqda.
• Yuqori samarali immunostimullovchi preparat “Timoptin” in’ektsiya uchun liofillangan kukun ishlab chiqarilmoqda. №5 flakonlarda 100 mkg dan (Institutda 2000 yildan beri ishlab chiqarilmoqda va O‘zbekiston Respublikasi dorixonalariga yetkazilmoqda);
• Tayanch-harakatlanish apparati yallig‘lanish kasalliklarini davolash uchun “Apikapsalvin” preparati tibbiyot amaliyotida qo‘llanilmoqda;
• “ Eritim 0,001% eritmasi” kamqonlikning yengil, o‘rta va og‘ir darajalarini davolash uchun eritropoez stimulyatori sifatida tibbiyot amaliyotida qo‘llanilmoqda (Institutda 2016 yildan beri ishlab chiqarilmoqda va O‘zbekiston Respublikasi dorixonalariga yetkazilmoqda).

 

Kelgusi rejalar:

• Aflan preparati bo‘yicha klinik sinovlar o‘tkazish va O‘zR FA Bioorganik kimyo Instituti bazasida ishlab chiqarishni tashkil etish.
• Kardin preparati bo‘yicha klinik sinovlar o‘tkazish va O‘zR FA Bioorganik kimyo Instituti bazasida ishlab chiqarishni tashkil etish.
• Sedanil preparati bo‘yicha klinik sinovlar o‘tkazish va ishlab chiqarishni tashkil etish.
• Biokor preparati bo‘yicha klinik sinovlar o‘tkazish va ishlab chiqarishni tashkil etish.

• barqarorlik namoyon qiluvchi, prolongirlangan ta’sirga ega, biomavjudligining samaradorligi orttirilgan yangi makromolekulyar terapevtik tizimlarni ishlab chiqish.
• yangi ko‘chirib o‘tkaziladigan xujayra kulturasi liniyalarini olish va ular asosida biologik faol moddalarni keng ko‘lamda skrining qilish uchun test tizim yaratish va ularning ta’sir mexanizmini o‘rganish

 

Ilmiy aloqalar:

RFA M.M.Shemyakin va Yu.A.Ovchinnikov nomidagi Bioorganik kimyo instituti;

RFA elementorganik birikmalar instituti; College Station, USA G‘o‘za patologiyasi bo‘limi; ARS Eyms Tuproq xo‘jaligi milliy laboratoriyasi, Ayova, AQSh;

XXR FA Shinjon fizika va kimyo texnika instituti;

Qishloq xo‘jaligi tadqiqotlari milliy instituti, Nant, Frantsiya. Stuttgart University,

Institute of Technical Biochemistry National Technical University of Athens, School of chemical engineering, Athens, Greece;

Food Materials Science Division, Institute of Food Research, Norwich Research Park, Colney, Norwich

 

Xalqaro loyihalar:

STCU Uz 001 “Investigation of Functionality Significant Proteins of Cotton Seeds and Development of Highly Effective Technologies of the Production of Provender on the Basis of Cotton Seed Oil Cake” 1999-2000 yy.

STCU Uz 75 “Development of biosensors for pesticides determination in environmental in cotton-cultivated countries” 2002-2003 yy.

STCU P 115 “Characterization of Phytoalexins from the Malvaceae in Uzbekistan” 2003-2006 yy.

M-11 “Markaziy Osiyo ba’zi yovvoyi o‘simliklari va zaharli hayvonlari biofaol peptidlari tadqiqi” O‘zbekiston-Rossiya loyihasi 2005-2006 yy.

STCU 3024 “O‘zbekistonda ajratib olingan propion bakteriyalarining biologik qiymatga ega tetrapirrollari  2005-2007 yy.

CRDF UZB2-31009-TA-08 “Parrandachilikda gossipol zaharliligini oldini olish uchun bentonit asosida ozuqa qo‘shimchasini yaratish 2008-2011 yy.

MR-38-2008 “O‘zbekiston endemik o‘simliklaridan yangi biologik faol moddalarni izlab topish O‘zbekiston-Rossiya loyihasi 2008-2009 yy.

INTAS-2000-00681 “Evaluation of the Role of Plant Cell Wall Degrading Enzymes on Yield and Quality of Oil Produced By Cold pressing-oil-producing crops” 2001-2003.

STCU P-119 “Development of Baculovirus-Based Bio-Insecticide Technology in Uzbekistan” 2003-2005.

INTAS Ref. № 05-1000005-7662 “Specific lipase catalyzed production of biodisel” 2006 – 2008

M–K–20 “Kam yog‘li o‘simlik urug‘laridan mahsulot unumi va biologik qiymati yuqori bo‘lgan yog‘ olishda polisaxaridaza fermentlari rolining tadqiqi va g‘o‘za chigitidan nativ gossipolni ajratib olish O‘zbekiston – Koreya 2008-2009 yy.

M–K–20-1”Aspergillus terreus zamburug‘i mahalliy shtammlarining polisaxaridaza fermentlari tadqiqi” O‘zbekiston – Koreya 2010—2011 yy.

 

Maqolalar:

1. Yu.I. Oщepkova, Ye.A. Rogojin, E.M. Sultanova, M.J. Oripoa, O.N. Veshkurova, S.A. Yegorov, Sh.I. Salixov Kationne peptid i belki iz semyan rasteniy semeystva Malvaceae // XPS № 2 2012 s.257-259

2. A.Yili, O.I. Ma, O.Y.Ly, Y.H.Gao, B.Zhao, O.N.Veshkurova, Sh.I.Salikhov, H,A,Alsa. Antioxidant peptides from Cicerarietinum of Xinjang China// XPS № 4 2012 s.578-580

3. V.V. Uzbekov, O.N. Veshkurova, et al. Quantitative analysis of antifeedant terpenoids of cotton cultivated in Uzbekistan// Chemistry of Natural Compounds, 2012, Volume 48, Issue 1 Page 153-154 DOI: 10.1007/s10600-012-0188-2

4. Munojat Oripova, Jamolitdin Ziyavitdinov, Juilia Oshepkova, Elvira Sultanova, Olga Veshkurova, Shodmon Namazov, Shavkat Salikhov// Comparative Characteristic Components of Plant Protective System by Example of Three Varieties of Cotton Plants.// The Asian and Australasian Journal of Plant Science and Biotechnology, Special Issue 2, 2013, p. 55-57

5. A.M. Asrorov, N. Sattorov, E.M. Sultanova, V.V. Uzbekov, Sh.T. Khodjayev, Sh.I. Salikhov Effects of different class’s insecticides on some secondary metabolites of cotton leaves The Asian and Australasian Journal of Plant Science and Biotechnology, Special Issue 1, 2013, pp. 43-47

6. Vyacheslav V. Uzbekov, Samat A. Talipov, Bakhtiyar T. Ibragimov, Robert D. Stipanovic and Alois A. Bell Lacinilene C 7-methyl ether(Structure Report)// Acta Crystallographica Section E (2013). E69, o1392 [doi:10.1107/S1600536813021430]

7. Vyacheslav V. Uzbekov, Samat A. Talipov, Bakhtiyar T. Ibragimov, Robert D. Stipanovic and Jinggao Liu Desoxyhemigossypol-6-methyl ether (Structure Report)//Acta Crystallographica Section E (2013). E69, o302 [doi:10.1107/S1600536813002304]

8. V.V. Uzbekov, R.D. Stipanovic, O.N. Veshkurova, S.E. Namazov, S.I. Salikhov Prediction of Cotton Resistance to Helicoverpa armigera Based on the (+)-Gossypol Content in Mature Seed // The Asian and Australasian Journal of Plant Science and Biotechnology 7 (Special Issue 2), 2013, p. 61-63

9. Akmal Asrorov, Elvira Sultanova, Olga Veshkurova, Navruz Sattarov, Shamil Khodjayev, Shavkat Salikhov Effects of Different Classes of Insecticides on the Activity of Hydrolyzes in Cotton Plant Leaves. Uzbekskiy Ximicheskiy Jurnal 1, 2013.

10. Akmal Asrorov, Elvira Sultanova, Olga Veshkurova, Navruz Sattarov, Shamil Khodjayev, Shavkat Salikhov Effects of Different Classes of Insecticides on the Activity of Oxidoreductase PR Proteins in Cotton Plant Leaves, Uzbekskiy Ximicheskiy Jurnal 2, 2013.

11. Yu.I. Oщepkova, O.N. Veshkurova, Sh.I. Salixov, D.V. Zaytsev, A.N. Smirnov, S.A. Yegorov, Ye.A. Rogojin Sravnitelny analiz ekstraktov semyan chernushki posevnoy (NIGELLA SATIVA), obladayuщix antifungalnoy aktivnostyu protivoomitseta PHYTOPHTHORA INFESTANS. Ximiya prirodnx soedineniy, 2013, №5, s.841-843.

12. M.J. Oripova, U.K. Ishimov, D.J. Ziyavitdinov, E.M. Sultanova, O.N. Veshkurova, Sh.E. Namazov, Sh.I. Salixov Sravnitelny analiz semyan nekotorx sortov i liniy xlopchatnika. Uzbekskiy Ximicheskiy Jurnal.2013 №6, s.42-45.

13. Beresneva Yu.V., Ibragimov F.A., Assesorova Yu.Yu. Protivoopuxolevaya i biologicheskaya aktivnost 5-ftoruratsila i soevogo belka, bogatogo izoflavonami na pozdnix srokax razvitiya opuxoli // «Inter-medikal», №1, 2014 g, s. 5-9.

14. Beresneva Yu.V., Ibragimov F.A., Vpova N.L. Vliyanie soevogo belka, bogatogo izoflavonami na model eksperimentalnogo tromboza levoy sonnoy arterii u krs v sravnenii s Nebiletom // Yevraziyskiy Soyuz Uchenx, 2014 g, №4, s. 22-26.

15. Oripova M.J., Sultanova E.M., Veshkurova O.N., E.M. Sultanova, V.V. Uzbekov, O.N. Veshkurova, Muxamedov I.M., Salixov Sh.I. Antimikrobnaya aktivnost peptidov prosvirnika Malvasylvestris. Vestnik NUUZ 2014 №3. s.14-16.

16. Akmal M. Asrorov, Haji Akbar Aisa, Abulimiti Yili, Ma Qing Ling, Olga Veshkurova, Elvira Sultanova, Shavkat Salikhov. Composite Insecticides Effects on Defensive and Nutritious Compounds in Cotton Leaves. Plant Biochemistry & Physiology. 2014, №1, pp.1-5.

17. A.M. Asrorov, J.F. Ziyavitdinov, U.J. Ishimov, E.M. Sultanova, O.N. Veshkurova, A.D. Matchanov, Sh.I. Salixov. Sravnitelnoe vliyanie razlichnx klassov insektitsidov na uroven svobodnx aminokislot, azota i kaliya v listyax xlopchatnika. Uzbekskiy Ximicheskiy Jurnal. 2014, №1, s.49-53

18. Akmal Asrorov, Navruz Sattarov, Olga Veshkurova, Abulimiti Yili, Elvira Sultanova, Haji Akbar Aisa, Shamil Khodjayev, Shavkat Salikhov. Cotton leaf urease and PAL activities, disulfide bonds under treatment with insecticides. International Journal of Plant Biology and Research. 2014, №1, pp. 1-5.

19. A. Yili, V.V. Maksimov, J.F. Ziyavitdinov, F.A. Ibragimov, Yu.V. Beresneva, Sh.I. Salixov, H.A. Aisa. Sravnitelnoe issledovanie polipeptidnogo sostava prorostkov Cicer Arietinum. Ximiya prirodnx soedineniy. 2014, № 5, c 573.

20. AA. Yili, V.V. Maksimov, Qing-Ling Ma, Yan-Hua Gao, O.N. Veshkurova, Sh.I. Salikhov, H.A. Aisa. Antimicrobial peptides from the plants. Journal of Pharmacy and Pharmacology, 2014, №2, s. 627-641.

21. Salixov Sh.I., Gafurova N.Dj., Vpova N.L., Ziyaeva A.V. Vliyanie preparata Kardin na gemostaz i nekotore bioximicheskie i gematologicheskie pokazateli krovi u krs pri eksperimentalnom arterialnom tromboze. Kardiologiya Uzbekistana, 2014, №3 (33), s.79-82

22. A. Waili, A. Yili, V.V. Maksimov, J.F. Ziyavitdinov, A. Mamadrahimov, O.N. Veshkurova, Sh.I. Salikhov, H.A. Aisa, Purification and Characterization of Trypsin Inhibitor from the Cicer arietinum L. (Chickpea) Sprouts, International Journal of Plant Biology and Research, 2015, Vol 3 (3), pp. 1040-1044.

23. A. Waili, A. Yili, V.V. Maksimov, J.F. Ziyavitdinov, A. Mamadrahimov, O.N. Veshkurova, Sh.I. Salikhov, H.A. Aisa, Purification and Characterization of Trypsin Inhibitor from the Cicer arietinum L. (Chickpea) Sprouts, Journal of Plant Biology Research,2015, №4, pp. 108-112.

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29. Akmal Asrorov, Ildiko Matusikova, Uchkun Ishimov, Jamolitdin Ziyavitdinov, Olga Veshkurova, Shavkat Salikhov, Comparative Analysis of Free Amino Acids and Nitrogen in Cotton Leaves Treated with Different Classes Insecticides. Agricultural Research. 2015, 4(3), rr.277–282

30. F.R. Muratova, X.K. Djalilov, Yu.I. Oщepkova, N.U. Yusupov, M.K. Xalmuratov, V.V. Uzbekov, E.M. Sultanova Izuchenie farmakokineticheskix svoystv Magnikoma // Farmatsevticheskiy vestnik Uzbekistana, 2016, № 1, s.64-68

31. N.U. Yusupov, Yu.I. Oщepkova, I.A. Xodjaeva, B.A. Imamaliev, A.B. Nigmatxodjaev, B.R. Mavlyanov Opredelenie bioekvivalentnost i isptuemogo preparata «Klotrimazol-RG» (klotrimazol) krem 2%, s preparatom-analogom «Klotrimazol» (klotrimazol) krem 2%, «Arpimed» SPAOZT, Respublika Armeniya //Farmatsevticheskiy vestnik Uzbekistana, 2016, № 1, s.79-82

32. Imamaliev B.A., Nigmatxodjaev A.B., Yusupov N.U, Yu.I.Oщepkova, Xodjaeva I.A., Shamsiev B., Shoislamov B.Sh., Mavlyanov B.R. Issledovanie farmakokineticheskix parametrov preparata «Entserta» //Farmatsevticheskiy vestnik Uzbekistana, 2016, №2, s.52-57

33. Nigmatxodjaev A.B., Yusupov N.U, Xodjaeva I.A., Imamaliev B.A., Yu.I.Oщepkova, Xolmuratov M.K., Shoislamov B.Sh. Metodika opredeleniya bioekvivalentnosti preparata Atsiklovir-RG (atsiklovir) maz //Farmatsevticheskiy vestnik Uzbekistana, 2016, №2, s.48-52

34. M.K. Xalmuratov, O.N. Veshkurova, N.U. Yusupov, E.M. Sultanova, Yu.I. Oщepkova, M.K. Salaxutdinova, I.R. Mavlyanov, A.Sh. Kasmov. Metodika opredeleniya bioekvivalentnosti lekarstvennogo preparata «Atsekard» v klinicheskix usloviyax //Farmatsevticheskiy vestnik Uzbekistana, 2016, №4, s.73-78

35. M.K. Xalmuratov, O.N. Veshkurova, E.M. Sultanova, Yu.I. Oщepkova, M.K. Salaxutdinova, N.U. Yusupov, B.R. Mavlyanov. Metodika opredeleniya bioekvivalentnosti preparata sulfokamfokain (sulfokamfornaya kislota & prokain) //Farmatsevticheskiy vestnik Uzbekistana, 2016, №4, s.78-81

36. A. Waili, A. Yili, V.V. Maksimov, Sh.I. Salikhov, H.A. Aisa. Isolation of Biologically Active Constituents from Fruit of Elaeagnus angustifolia. Chemistry of Natural Compounds, 2016, Vol. 52, № 3, pp. 574- 576.

37. A. Waili, A. Yili, V.V. Maksimov, Sh.I. Salixov. Vdelenie biologicheski aktivnx veщestv iz Elaeagnus angustifolia. XPS, 2016, № 3, s. 495- 496.

38. Bekchanov D. J., Sagdiev N.G., Mukhamediev M. G. Study Sorption Of Heavy Metals Nitrogen And Phosphorus Containing Polyampholytes // American Journal of Polymer Science, 2016; № 6(2),r. 46-49.

39. Bekchanov D.J., Sagdiev N.J., Muxamediev M.G. Sintez i fiziko-ximicheskie svoystva anionoobmennogo sorbenta na osnove polivinilxlorida// Universum: Ximiya i biologiya, 2016, № 10 (28), s. 36 – 40

40. Baxramov S.M., Suleymanova D.N., Babadjanova Sh.A., Salixov Sh.I., Gafurova N.Dj., Xamidov Sh.A., Iskanderova S.Dj. Effektivnost preparata Eritim v lechenii bolnx jelezodefitsitnoy anemiey. FarmatsevticheskiyvestnikUzbekistana, №1, 2016, s.72-78.

41. Y. Mijiti, P. Rozi, A. Waili, Y.H. Gao, V.V. Maksimov, J.F. Ziyavitdinov, A. Yili, Sh.I. Salixov, H.A. Aisa. Vdelenie antimikrobnx peptidov iz Fritillaria palladiflora Schrenk, XPS, 2017, № 6, s. 972-975.

42. Bekchanov D.J., Sagdiev N.J., Juraev M.M., Muxamediev M.G.Kinetika i termodinamika protsessa sorbtsii organicheskix kislot sorbentom na osnove polivinilxlorida // Universum: Ximiya i biologiya, № 10 (40), g. 2017, str. 17 – 21

43. Yuilia Oshchepkova Creation of the wound healing pharmaceutical composition // Medicine and Medical Sciences 3(3): 013-017, August 2017 DOI: 10.15413/mms.2017.0400

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45. Yu.V. Beresneva, F.A. Ibragimov, M.S. Gildieva The role of biologically active components from soybeans in enhancement of the effectiveness of chemo- and radiotherapy of transplanted tumors //Uzbek biological journal, 2017, №5, R.7-10.

46. A.M. Asrorov, U.J. Ishimov, E.M. Sultanova, Yu.I. Oshepkova, J.F.Ziyavitdinov, O.N. Veshkurova, Sh.I. Salikhov Treatment with insecticides affects cotton plant’s aphid resistance// Uzbek biological journal, 2017, №5, R.3-7.

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49. Iskanderova S.Dj., Mamanazarova D.K., Maksudova M.B., Tursunbaev R.S. Kompleksnoe lechenie anemii pri revmatoidnom artrite s primeneniem otechestvennogo antianemicheskogo preparata Eritim. Terapevticheskiy vestnik Uzbekistana, 2017, № 4, s.80-85.

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53. D.E. Dusmatova, V.V. Uzbekov, R.F. Muxamatxanova, N.Yu. Beshko, L.T. Djuraeva Kolichestvennoe opredelenie seskviterpenovx laktonov britanina i inuchinenolida S v tsvetkax devyasila britanskogo Inula Britannica L. metodom VEJX // Universum: ximiya i biologiya, №7 (49), 2018. URL: http://7universum.com/ru/nature/archive/item/6089

54. Beresneva Yu.V., Ibragimov F.A., Vpova N.L. Izmenenie sostoyaniya mineralnogo obmena kostnoy tkani preparatom Soyaflan pri eksperimentalnom osteoporoze // Vestnik biotexnologii i fiziko-ximicheskoy biologii im. Yu.A. Ovchinnikova, 2018, t.14, №2, s. 54-59.

 

Patentlar:

1. A. Yili, H.A. Aisa, Aihemiding Waili, Shavkat I. Salikhov A method of preparation of chickpea polypeptides fractions and its application, Patent Application number: 201510191380.6; China.

2. Patent na izobretenie №IAP 04601 ot 12.10.2010 Sposob otsenki rezistentnosti sort obraztsov xlopchatnika k xlopkovoy sovke (Uzbekov V.V., Veshkurova O.N., Abduraximov R.Sh., Salixov Sh.I., Namazov Sh.E., Amanturdiev I.G.)

3. Patent na izobretenie №IAP 04614 ot 12.10.2010 Defenzin, obladayuщiy antimikrobnoy aktivnostyu (Oщepkova Yu.I., Rogojin Ye.A., Veshkurova O.N., Salixov Sh.I., Yegorov S.A., Grishin Ye.V.)

4. Patent na izobretenie №IAP 05240 ot 19.06.2013 Farmatsevticheskaya ranozajivlyayuщaya kompozitsiya (Oщepkova Yu.I., Kazantseva D.S., Veshkurova O.N., Salixov Sh.I.)

5. Patent na izobretenie №IAP 05258 ot 20.09.2013 Sposob polucheniya immunostimuliruyuщix timusnx polipeptidov, vliyayuщix na gemopoez (Salixov Sh.I., Gafurova N.Dj., Xamidov Sh.A., Vpova N.L.)

6. Patent na izobretenie №IAP 05428 ot 11.07.2014Shtamm kultiviruemx kletok raka tonkoy kishki mshey AKAT, klon Ye7(Xashimova Z.S., Kaxarova K.A., Sagdiev N.J., Ibragimov F.A., Beresneva Yu.V., Turaev A.S., Salixov Sh.I)

7. Zayavka na patent № IAP 2018 0267 ot 12.06.2018 Sposob polucheniya rekombinantnoy form defenzina (Oщepkova Yu.I., Salixov Sh.I., Soy V.E., Turdikulova Sh.U., A. Yili, X.A. Aysa)

 

Metodik yo‘riqnomalar:

1. V.V. Uzbekov, A.M. Asrorov, E.M. Sultanova, O.N. Veshkurova, Sh.I. Salixov “Metodicheskie rekomendatsii po kolichestvennoy otsenke urovnya vtorichnx metabolitov, opredelyayuщix ustoychivost xlopchatnika v norme i posle yego obrabotki insektitsidami” // IBOX AN RUz, 2014

2. Ibragimov F.A., Assesorova Yu.Yu., Beresneva Yu.V., Kireev G.V. Izuchenie protivoopuxolevoy aktivnosti farmakologicheskix veщestv, orientirovannx na primenenie v klinike solidnx novoobrazovaniy // Metodicheskie rekomendatsii, Tashkent, 2014 g, s.20.